MCB6175C: Integrative Microscopy 3
The theoretical aspects of Bright Field, Dark Field, Phase Contrast, Fluorescence, Confocal and Electron Microscopy will be discussed. Mastery of specimen preparation techniques, thick and thin sectioning, special staining, freeze fracture, enzyme histochemistry, immunolabelling, and heavy metal staining will be accomplished. These advanced techniques will allow students to observe cellular structure and ultrastructure, while the use of specific labels to probe for macromolecules will emphasize the link between structure and function in biology. (A laboratory fee of $51.93 will be assessed.)