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Chemistry Faculty: Bryan Knuckley, Ph.D.

Dr. Bryan Knuckley headshot

Associate Professor of Chemistry

Office: Science & Engineering (Building 50), Room 3528

Phone: (904) 620-1918

Email: bryan.knuckley@unf.edu

Education

Bachelor of Science - University of South Carolina 2003

Ph.D. - University of South Carolina 2009

Postdoctoral Research - The Scripps Research Institute 2010-2012

Teaching

Dr. Knuckley teaches General Chemistry I Lecture and Laboratory, and Biochemistry.

Research

Dr. Knuckley is interested in understanding the substrate specificity, physiological, and pathological roles of a family of proteins called the Protein Arginine Methyltransferases (PRMTs). These nine mammalian enzymes (PRMT 1-9) catalyze the addition of methyl groups on arginine residues within proteins. Dysregulated arginine methylation of proteins has a significant role in the onset and progression of numerous human diseases (i.e., cardiovascular disease, prostate cancer, breast cancer, etc.), which has contributed to these proteins becoming an important therapeutic target. The Knuckley Lab is interested in developing novel methods to characterize the differences in substrate specificity within this family in a high-throughput fashion. Identification of key factors that contribute to the substrate specificity will allow for the development of novel therapeutics.

Dr. Knuckley is also interested in studying the catalytic mechanism of the guanidinium-modifying enzymes (GME) superfamily. A specific member of the GME family, the agmatine deiminase (AgD) is expressed in many pathogenic bacteria. Agmatine deiminase is responsible for converting agmatine to N-¬carbamoylputrescine with concurrent release of ammonia. This enzyme is believed to play a role in the acid resistance of these bacteria and enhance the innate immune response.

Funding

  • University of North Florida, Scholars Transforming Academic Research, 2015
  • University of North Florida, Faculty Development Scholarship Grant, 2015
  • University of North Florida, College of Arts and Sciences Deans Leadership Fellowship Grant, 2013
  • University of North Florida, Faculty Development Scholarship Grant, 2013

Recent Publications

"Mechanistic Studies on the Agmatine Deiminase from Listeria monocytogenes," Soares, C. A. and Knuckley, B.*, Biochemical Journal, 473, 1553 - 1561 (2016). http://dx.doi.org/10.1042/BCJ20160221

 

Agmatine Deiminase from Listeria monocytogenes - more info found within report

 

"Development of a Plate-Based Screening Assay to Investigate the Substrate Specificity of the PRMT Family of Enzymes," Nguyen, H. C., Wang, M., Salsburg, A., Knuckley, B.*, ACS Combinatorial Science, 17, 500-505 (2015). http://dx.doi.org/10.1021/acscombsci.5b00070

 

Development of a Plate-Based Screening Assay - more info found within report

 

"Development of a clickable activity-based protein profiling (ABPP) probe for agmatine deiminases," Marchenko, M., Thomson, A., Ellis, Terri N., Knuckley, B.*, and Causey, C.P*,. Bioorganic and Medicinal Chemistry, (23)9, 2159-2167 (2015). http://dx.doi.org/10.1016/j.bmc.2015.03.013

 

Development of a clickable activity-based protein profiling - more info found within report

 

"Design, synthesis, and in vitro evaluation of an activity-based protein profiling (ABPP) probe targeting agmatine deiminases," Thomson, A., O'Connor, S., Knuckley, B., and Causey, C.P*, Bioorganic and Medicinal Chemistry, 22(17), 4602-4608, (2014). http://dx.doi.org/10.1016/j.bmc.2014.07.028

 

Design, synthesis, and in vitro evaluation - more info found within report

 

"Mechanistic studies of Protein Arginine Deiminase 2: Evidence for a substrate-assisted mechanism," Dreyton, C.J., Knuckley, B., Jones, J.E., Lewallen, D.M., Thompson, P.R*, Biochemistry, 53, 4426-4433, (2014). http://dx.doi.org/10.1021/bi500554b

 

Mechanistic studies of Protein Arginine Deiminase 2 - more info found within report