Amy L Lane

Assistant Professor

Chemistry • College of Arts & Sciences

Areas of Expertise

Natural products, also known as secondary metabolites, are organic molecules without a recognizable function in growth, development, or reproduction of the producing organism. Research in a range of interconnected scientific fields has resulted in discovery of unusual secondary metabolites with immense clinical utility, development of nature-inspired organic synthesis routes, understanding of genes directing biosynthesis of natural products, and insights into intriguing enzymes employed in formation of these molecules.

Dr. Amy Lane’s group is carrying out both basic and applied scientific studies on a variety of topics related to natural products.
Our major interests include:
• Exploration of understudied genera of marine microorganisms as sources of new human drugs. We are especially interested in discovering new compounds with potential utility as antibacterial (antibiofilm) or anticancer drugs. We are tackling this challenge using a combined chemical-genetic approach, employing genetic information from marine microorganisms as a tool for guiding discovery of new natural products.
• Probing the ecological function of marine microbial natural products. While the biomedical utility of marine natural products is widely recognized, the basic ecological functions of these compounds are far less explored. We are working to understand the fundamental adaptive roles played by compounds produced by marine organisms, with a special focus on chemically-mediated interactions among marine microorganisms.
• Revealing the genetic and enzymatic basis for biosynthesis of structurally unusual marine natural products. This knowledge holds great potential for development of chemoenzymatic syntheses and fully exploiting the potential of marine microorganisms as sources of biologically active molecules.

For more information about research interests of the Lane Lab and to learn how to join the team, check out http://www.unf.edu/~amy.lane
 

Education

Bachelor of Science (Summa Cum Laude) - Indiana State University 2003
Ph.D. - Georgia Institute of Technology 2008 - Organic/Biochemistry
Postdoctoral Research - Scripps Institution of Oceanography at University of California - San Diego 2008-2010

Biography

Awards

--2014 UNF Outstanding Undergraduate Teaching Award
--2014 Gordon Research Conference Primarily Undergraduate Institution Travel Award
--2013 Mentor of the Year Award at UNF (one campus-wide award each year)
--2012 Nominee, Mentor of the Year Award at UNF (14 campus-wide nominees)
--2011, 2012 UNF Disability Resource Center Professor Empowerment Award
 

Affiliations

--Courtesy Appointment in UNF Biology Department.  

--Research Collaborator Appointment at Mayo Clinic Jacksonville (Department of Cancer Biology).

Grants and Contracts Awarded

External Funding @ UNF:

--2014 National Institutes of Health Southeast Center for Integrated Metabolomics Feasibility and Pilot Grant, Principle Investigator (PI), Microbial competition as an activator of natural product production. 

--2013 Florida Institute of Oceanography 4-day Ship Time Award, PI with Dr. Cliff Ross of UNF Biology.  Biofilm inhibitors from marine microorganisms.

--2012-2014 Research Corporation Cottrell College Science Award, PI.  A genetic-chemical approach for exploring biotic interactions as activators of natural product biosynthesis among marine bacteria.

--2012-2013 American Society of Pharmacognosy Research Starter Grant, PI.  Marine natural product modulators of fungal biofilms.

--2011 Florida Institute of Oceanography 7-day Ship Time Award, PI with Dr. Cliff Ross of UNF Biology.  Exploration of marine actinomycete bacteria of the Dry Tortugas and Marquesas Keys as sources of novel human antibiotics

 

Internal Funding @ UNF:

--2014-2015 University of North Florida, Transformative Learning Opportunity Grant.  Diving into marine natural products: Immersing undergraduates in biomedical research

--2014 University of North Florida, Academic Affairs, Proposal Development Grant.  Development of a National Science Foundation Research at Undergraduate Institutions (NSF-RUI) grant

--2013 University of North Florida College of Arts and Sciences, Faculty Enhancement Plan Grant.  Microbial competition as an activator of natural product production

--2013 University of North Florida Environmental Center Seed Grant (with Dr. Thomas J. Mullen of UNF Chemistry).  Natural products as environmentally friendly inhibitors of aquatic biofilms

--2012 University of North Florida College of Arts and Sciences, Faculty Enhancement Plan Grant (with Dr. Gregory Ahearn of UNF Biology).  Heavy metals stimulate essential amino acid absorption across crustacean digestive tract by using molecular mimicry and a peptide transport protein

--2012 University of North Florida, Academic Affairs, Scholarship Grant.  Marine natural products as bacterial biofilm inhibitors.

--2012-2013 University of North Florida, Transformative Learning Opportunity Grant.  Marine microorganisms of northeast Florida as sources of potential diabetes therapeutics.

--2012 University of North Florida, Dean’s Council Faculty Fellowship.  Marine organisms as sources of novel biofilm inhibiting molecules.

--2011-2012 University of North Florida, Transformative Learning Opportunity Grant.  Diving for drugs: Exploration of Florida Keys marine microbes as a source of novel antibiotics.

--2011 University of North Florida, Academic Affairs, Proposal Development Grant.  Development of an NIH AREA (R15) grant for discovery of new antibiotic compounds from marine microbes.  
 

Publications & Presentations

18. Peterson M, Lane AL, Ahearn GA. Analysis of glycylsarcosine transport by lobster intestine using gas chromatography. Journal of Comparative Physiology – B. Submitted.

17. Xu M, Hillwig ML, Lane AL, Tiernan MS, Moore BS, Peters RJ. Characterization of an orphan diterpenoid biosynthetic operon from Salinispora arenicola. Journal of Natural Products. Submitted.

16. Lane AL, Mandelare PE, Ban YH. New developments in NMR methodologies with special roles in the drug discovery process. In: Structure-Activity Relationship Studies in Drug Development by NMR Spectroscopy, Volume II. Choudhary AUR and Iqbal M, Eds. Bentham eBook. Accepted.

15. Kersten R, Lane AL, Nett M, Richter TKS, Duggan BM, Dorrestein PC, Moore BS. (2013) Bioactivity-guided genome mining reveals the lomaiviticin biosynthetic gene cluster in Salinispora tropica. ChemBioChem. 14: 955-962.

14. Lane AL, Nam SJ, Fukuda T, Yamanaka K, Kauffman CA, Jensen PR, Fenical W, Moore BS. (2013) Structures and comparative characterization of biosynthetic gene clusters for cyanosporasides, enediyne-derived natural products from marine actinomycetes. Journal of the American Chemical Society. 135: 4171-4174.

13. Abdul-Hay SO, Lane AL, Caulfield TR, Claussin C, Bertrand J, Masson A, Choudhry S, Fauq AH, Maharvi GM, Leissring MA. (2013) Optimization of peptide hydroxamate inhibitors of insulin-degrading enzyme reveals marked substrate selectivity. Journal of Medicinal Chemistry. 56: 2246-2255.

12. Lane AL, Moore BS. (2011) A sea of biosynthesis: Marine natural products meet the molecular age. Natural Product Reports. 28: 411-428.

11. Lane AL, Mular L, Drenkard EJ, Shearer TL, Engel S, Fredericq S, Fairchild CR, Prudhomme J, Le Roch K, Hay ME, Aalbersberg W, Kubanek J. (2010) Ecological leads for natural product discovery: Novel sesquiterpene hydroquinones from the red alga Peyssonnelia sp. Tetrahedron. 66: 455-461.

10. Whalen KE, Lane AL, Kubanek J, Hahn ME. (2010) Biochemical warfare on the reef: The role of glutathione transferases in consumer tolerance of dietary prostaglandins. PLoS One. 5(1): e8537.

9. Lane AL, Nyadong L, Galhena AS, Shearer TL, Stout EP, Parry RM, Kwasnik M, Wang M, Hay ME, Fernandez FM, Kubanek J. (2009) Desorption electrospray ionization mass spectrometry reveals surface-mediated antifungal chemical defense of a tropical seaweed. Proceedings of the National Academy of Sciences. 106: 7314-7319.
Cover feature: “Seaweed employs distributed chemical defense.” http://www.pnas.org/content/106/18/7265
Commentary: http://www.pnas.org/content/106/18/7269.extract

8. Nyadong L, Hohenstein EG, Galhena AS, Lane AL, Kubanek J, Sherrill CD, Fernandez FM. (2009) Reactive desorption electrospray ionization mass spectrometry (DESI MS) of natural products of a marine alga. Analytical and Bioanalytical Chemistry. 394: 245-254

7. Lane AL, Stout EP, Lin AS, Prudhomme J, Le Roch K, Fairchild CR, Franzblau SG, Hay ME, Aalbersberg W, Kubanek J. (2009) Antimalarial bromophycolides J-Q from the Fijian red alga Callophycus serratus. Journal of Organic Chemistry. 74: 2736-2742.

6. Stout EP, Hasemeyer A, Lane AL, Davenport T, Engel S, Hay ME, Fairchild CR, Prudhomme J, Le Roch K, Aalbersberg W, Kubanek J. (2009) Antibacterial neurymenolides from the Fijian red alga Neurymenia fraxinifolia. Organic Letters. 11: 225-228.

5. Lane AL, Kubanek J. (2008) Secondary metabolite defenses against pathogens and biofoulers. Invited review. In Algal Chemical Ecology. C.D. Amsler, Ed. Springer-Verlag. pp. 229-243.

4. Jiang RW, Lane AL, Mylacraine L, Hardcastle KI, Fairchild CR, Aalbersberg W, Hay ME, Kubanek J. (2008) Structures and absolute configurations of sulfate-conjugated triterpenoids including an antifungal chemical defense of the green macroalga Tydemania expeditionis. Journal of Natural Products. 71: 1616-1619.

3. Lane AL, Stout EP, Hay ME, Prusak AC, Hardcastle K, Fairchild CR, Aalbersberg W, Kubanek J. (2007) Callophycoic acids and callophycols from the Fijian red alga Callophycus serratus. Journal of Organic Chemistry. 72: 7343-7351.

2. Lane AL, Kubanek J. (2006) Structure-activity relationship of chemical defenses from the freshwater plant Micranthemum umbrosum. Phytochemistry. 67: 1224-1231.

1. Kolodny NH, Bauer D, Bryce K, Klucevsek K, Lane A, Medeiros L, Mercer W, Moin S, Park D, Peterson J, Wright J, Yuen C, Wolfson AJ, Allen MM. (2006) Effect of nitrogen source on cyanophycin synthesis in Synechocystis sp. strain PCC 6308. Journal of Bacteriology. 188: 934-940.
 

LaneContact Information

Building 50, Room 3518

(904) 620-2315

amy.lane@unf.edu

Website