Office: Science & Engineering (Building 50), Room 3528
Phone: (904) 620-1951
Bachelor of Science - University of South Carolina 2003
Ph.D. - University of South Carolina 2009
Postdoctural Research - The Scripps Research Institute 2010-2012
Dr. Knuckley teaches General Chemistry I Lecture and Laboratory.
Dr. Knuckley is interested in understanding the substrate specificity, physiological, and pathological roles of a family of proteins called the Protein Arginine Methyltransferases (PRMTs). These nine mammalian enzymes (PRMT 1-9) catalyze the addition of methyl groups on arginine residues within proteins. Dysregulated arginine methylation of proteins has a significant role in the onset and progression of numerous human diseases (i.e., cardiovascular disease, prostate cancer, breast cancer, etc.), which has contributed to these proteins becoming an important therapeutic target. The Knuckley Lab is interested in developing novel methods to characterize the differences in substrate specificity within this family in a high-throughput fashion. Identification of key factors that contribute to the substrate specificity will allow for the development of novel therapeutics.
Dr. Knuckley is also interested in studying the catalytic mechanism of the guanidinium-modifying enzymes (GME) superfamily. A specific member of the GME family, the agmatine deiminase (AgD) is expressed in many pathogenic bacteria. Agmatine deiminase is responsible for converting agmatine to N-¬carbamoylputrescine with concurrent release of ammonia. This enzyme is believed to play a role in the acid resistance of these bacteria and enhance the innate immune response.
“Development of a Plate-Based Screening Assay to Investigate the Substrate Specificity of the PRMT Family of Enzymes,” Nguyen, H. C., Wang, M., Salsburg, A., Knuckley, B.*, ACS Combinatorial Science, 17, 500-505 (2015). http://dx.doi.org/10.1021/acscombsci.5b00070
“Development of a clickable activity-based protein profiling (ABPP) probe for agmatine deiminases,” Marchenko, M., Thomson, A., Ellis, Terri N., Knuckley, B.*, and Causey, C.P*,. Bioorganic and Medicinal Chemistry, (23)9, 2159-2167 (2015). http://dx.doi.org/10.1016/j.bmc.2015.03.013
“Design, synthesis, and in vitro evaluation of an activity-based protein profiling (ABPP) probe targeting agmatine deiminases,” Thomson, A., O’Connor, S., Knuckley, B., and Causey, C.P*, Bioorganic and Medicinal Chemistry, 22(17), 4602-4608, (2014). http://dx.doi.org/10.1016/j.bmc.2014.07.028
“Mechanistic studies of Protein Arginine Deiminase 2: Evidence for a substrate-assisted mechanism,” Dreyton, C.J., Knuckley, B., Jones, J.E., Lewallen, D.M., Thompson, P.R*, Biochemistry, 53, 4426-4433, (2014). http://dx.doi.org/10.1021/bi500554b
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